Article
Direct monitoring of allosteric recognition of type IIE restriction endonuclease EcoRII.
The Journal of biological chemistry - 30 May 2008
Takahashi Shuntaro, Matsuno Hisao, Furusawa Hiroyuki, Okahata Yoshio
Abstract excerpt
EcoRII is a homodimer with two domains consisting of a DNA-binding N terminus and a catalytic C terminus and recognizes two specific sequences on DNA. It shows a relatively complicated cleavage reaction in bulk solution. After binding to either recognition site, EcoRII cleaves the other recognition site of the same DNA (cis-binding) strand and/or the recognition site of the other DNA (trans-binding) strand....
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