Article
Localization of a protein-DNA interface by random mutagenesis.
The EMBO journal - 1 Dec 1998
O'Neill M, Dryden D T, Murray N E
Abstract excerpt
The type I restriction and modification enzymes do not possess obvious DNA-binding motifs within their target recognition domains (TRDs) of 150-180 amino acids. To identify residues involved in DNA recognition, changes were made in the amino-TRD of EcoKI by random mutagenesis. Most of the 101 sub...
Topics
- Amino Acid Sequence
- DNA
- DNA Restriction Enzymes
- Electrophoresis, Agar Gel
- Escherichia coli
- Fluorescence Polarization
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis
- Phenotype
- Protein Conformation
- S-Adenosylmethionine
- Site-Specific DNA-Methyltransferase (Adenine-Specific)