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Article

Localization of a protein-DNA interface by random mutagenesis.

The EMBO journal - 1 Dec 1998

O'Neill M, Dryden D T, Murray N E

Abstract excerpt

The type I restriction and modification enzymes do not possess obvious DNA-binding motifs within their target recognition domains (TRDs) of 150-180 amino acids. To identify residues involved in DNA recognition, changes were made in the amino-TRD of EcoKI by random mutagenesis. Most of the 101 substitutions affecting 79 residues had no effect on the phenotype. Changes at only seven positions caused the loss of...

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Identifiers

PMID: 9843515
DOI: 10.1093/emboj/17.23.7118
PMCID: PMC1171058

Genes

TRD

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Source receipt: pubmed, retrieved 7 Sept 2026.

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