Article
DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme.
Nucleic acids research - 1 Nov 2010
Zaremba Mindaugas, Owsicka Amelia, Tamulaitis Gintautas, Sasnauskas Giedrius, Shlyakhtenko Luda S, Lushnikov Alexander Y, Lyubchenko Yuri L, Laurens Niels, van den Broek Bram, Wuite Gijs J L, Siksnys Virginijus
Abstract excerpt
To cut DNA at their target sites, restriction enzymes assemble into different oligomeric structures. The Ecl18kI endonuclease in the crystal is arranged as a tetramer made of two dimers each bound to a DNA copy. However, free in solution Ecl18kI is a dimer. To find out whether the Ecl18kI dimer or tetramer represents the functionally important assembly, we generated mutants aimed at disrupting the putative...
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