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Article

Role of DNA minor groove interactions in substrate recognition by the M.SinI and M.EcoRII DNA (cytosine-5) methyltransferases.

Nucleic acids research - 1 Aug 2001

Kiss A, Pósfai G, Zsurka G, Raskó T, Venetianer P

Abstract excerpt

The SinI and EcoRII DNA methyltransferases recognize sequences (GG(A)/(T)CC and CC(A)/(T)GG, respectively), which are characterized by an (A)/(T) ambiguity. Recognition of the A.T and T.A base pair was studied by in vitro methyltransferase assays using oligonucleotide substrates containing a hypoxanthine.C base pair in the central position of the recognition sequence. Both enzymes methylated the substituted...

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Identifiers

PMID: 11470876
DOI: 10.1093/nar/29.15.3188
PMCID: PMC55819

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Source receipt: pubmed, retrieved 7 Sept 2026.

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