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Article

Alternative arrangements of catalytic residues at the active sites of restriction enzymes.

FEBS letters - 8 May 2002

Tamulaitis Gintautas, Solonin Alexander S, Siksnys Virginijus

Abstract excerpt

A catalytic sequence motif PDX10-30(E/D)XK is found in many restriction enzymes. On the basis of sequence similarities and mapping of the conserved residues to the crystal structure of NgoMIV we suggest that residues D160, K182, R186, R188 and E195 contribute to the catalytic/DNA binding site of the Ecl18kI restriction endonuclease. Mutational analysis confirms the functional significance of the conserved...

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PMID: 11997010
DOI: 10.1016/s0014-5793(02)02621-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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