Article
Structural and chemical basis for glucosamine 6-phosphate binding and activation of the glmS ribozyme.
Biochemistry - 21 Apr 2009
Cochrane Jesse C, Lipchock Sarah V, Smith Kathryn D, Strobel Scott A
Abstract excerpt
The glmS ribozyme is the first naturally occurring catalytic RNA that relies on an exogenous, nonnucleotide cofactor for reactivity. From a biochemical perspective, the glmS ribozyme derived from Bacillus anthracis is the best characterized. However, much of the structural work to date has been done on a variant glmS ribozyme, derived from Thermoanaerobacter tengcongensis. Here we present structures of the B....
Topics
- Bacillus anthracis
- Bacterial Proteins
- Binding, Competitive
- Catalysis
- Crystallography, X-Ray
- Enzyme Activation
- Glucosamine
- Glucose-6-Phosphate
- Kinetics
- Ligands
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- RNA, Catalytic
- Structure-Activity Relationship