Article
Regulated high-level expression of the mannitol permease of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
van Weeghel R P, Keck W, Robillard G T
Abstract excerpt
The structural gene (mtlA) of the Escherichia coli phosphoenolpyruvate-dependent mannitol-transport protein (EIImtl) and its upstream promoter region (Pmtl) were subcloned approximately 150 base pairs downstream of a lambda PR promoter on a multicopy mutagenesis/expression vector and used to tran...
Topics
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genotype
- Microscopy, Electron
- Monosaccharide Transport Proteins
- Phenotype
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Plasmids
- Restriction Mapping