Article
Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices.
The Journal of biological chemistry - 5 Dec 1992
Webb D C, Rosenberg H, Cox G B
Abstract excerpt
The Escherichia coli Pst system is a periplasmic phosphate permease. A mutational analysis of the requirement for function of specific charged residues or proline residues in the two hydrophobic subunits (PstC and PstA) has been carried out. No residues, among 19 charged residues altered, were found to be essential for phosphate uptake, although some alterations resulted in partial effects. Evidence was obtained...
Topics
- ATP-Binding Cassette Transporters
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Cell Membrane
- Escherichia coli
- Escherichia coli Proteins
- Genotype