Article
Phosphorylation site mutants of the mannitol transport protein enzyme IImtl of Escherichia coli: studies on the interaction between the mannitol translocating C-domain and the phosphorylation site on the energy-coupling B-domain.
Biochemistry - 14 Mar 1995
Boer H, ten Hoeve-Duurkens R H, Lolkema J S, Robillard G T
Abstract excerpt
Mannitol binding and translocation catalyzed by the C domain of the Escherichia coli mannitol transport protein enzyme IImtl is influenced by domain B. This interaction was studied by monitoring the effects of mutating the B domain phosphorylation site, C384, on the kinetics of mannitol binding t...
Topics
- Base Sequence
- Binding Sites
- Biological Transport, Active
- DNA Primers
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Hydrogen-Ion Concentration
- Kinetics
- Mannitol
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutagenesis, Site-Directed
- Mutation