Article
Molecular cloning of the C-terminal domain of Escherichia coli D-mannitol permease: expression, phosphorylation, and complementation with C-terminal permease deletion proteins.
Journal of bacteriology - 1 Mar 1990
White D W, Jacobson G R
Abstract excerpt
We have subcloned a portion of the Escherichia coli mtlA gene encoding the hydrophilic, C-terminal domain of the mannitol-specific enzyme II (mannitol permease; molecular mass, 68 kilodaltons [kDa]) of the phosphoenolpyruvate-dependent carbohydrate phosphotransferase system. This mtlA fragment, e...
Topics
- Chromosome Deletion
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Fermentation
- Gene Expression
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Kinetics
- Models, Biological
- Monosaccharide Transport Proteins
- Phosphoenolpyruvate
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Phosphorylation
- Plasmids