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Article

Using affinity chromatography to engineer and characterize pH-dependent protein switches.

Protein science : a publication of the Protein Society - 1 Jan 2009

Sagermann Martin, Chapleau Richard R, DeLorimier Elaine, Lei Margarida

Abstract excerpt

Conformational changes play important roles in the regulation of many enzymatic reactions. Specific motions of side chains, secondary structures, or entire protein domains facilitate the precise control of substrate selection, binding, and catalysis. Likewise, the engineering of allostery into proteins is envisioned to enable unprecedented control of chemical reactions and molecular assembly processes. We here...

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Identifiers

PMID: 19177365
DOI: 10.1002/pro.23
PMCID: PMC2708033

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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