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Article

An enzyme-based biosensor for monitoring and engineering protein stability in vivo.

Proceedings of the National Academy of Sciences of the United States of America - 30 Mar 2021

Ren Chang, Wen Xin, Mencius Jun, Quan Shu

Abstract excerpt

Protein stability affects the physiological functions of proteins and is also a desirable trait in many protein engineering tasks, yet improving protein stability is challenging because of limitations in methods for directly monitoring protein stability in cells. Here, we report an in vivo stability biosensor wherein a protein of interest (POI) is inserted into a microbial enzyme (CysGA) that catalyzes the...

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Identifiers

PMID: 33753520
DOI: 10.1073/pnas.2101618118
PMCID: PMC8020752

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