Engineering the native ensemble to tune protein function: Diverse mutational strategies and interlinked molecular mechanisms.

Abstract excerpt

Natural proteins are fragile entities, intrinsically sensitive to perturbations both at the level of sequence and their immediate environment. Here, we highlight the diverse strategies available for engineering function through mutations influencing backbone conformational entropy, charge-charge interactions, and in the loops and hinge regions, many of which are located far from the active site. It thus appears...

Topics

• Protein Engineering
• Mutation
• Proteins
• Protein Conformation
• Humans
• Models, Molecular
• Thermodynamics
• Protein Folding
• Allosteric Regulation