Article
Parallel activation of the NIMA and p34cdc2 cell cycle-regulated protein kinases is required to initiate mitosis in A. nidulans.
Cell - 18 Oct 1991
Osmani A H, McGuire S L, Osmani S A
Abstract excerpt
We show that in Aspergillus nidulans, p34cdc2 tyrosine dephosphorylation accompanies activation of p34cdc2 as an H1 kinase at mitosis. However, the nimA5 mutation arrests cells in G2 with p34cdc2 tyrosine dephosphorylated and fully active as an H1 kinase. Activation of NIMA is therefore not required for p34cdc2 activation. Furthermore, mutation of nimT, which encodes a protein with 50% similarity to fission yeast...
Topics
- Aspergillus nidulans
- Benomyl
- Blotting, Western
- CDC2 Protein Kinase
- Enzyme Activation
- Kinetics
- Mitosis
- Mutation
- Phosphorylation
- Protein Kinases
- Temperature
- Tyrosine