Article
Contribution of individual amino acids to the RNA binding activity of the Wilms' tumor suppressor protein WT1.
Biochemistry - 13 Jan 2009
Weiss Tristen C, Romaniuk Paul J
Abstract excerpt
In addition to binding to DNA, the zinc finger protein WT1 can also bind specifically to RNA. To determine the role of individual zinc fingers of the protein in this RNA binding activity, deletion and substitution mutants of the WT1 zinc finger domain were constructed. The effects of the various mutations on the binding of WT1 to the RNA aptamers RNA22 and RNA38 were determined using a quantitative equilibrium...
Topics
- Amino Acids
- Aptamers, Nucleotide
- DNA
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- RNA
- WT1 Proteins
- Zinc Fingers