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Article

A genetic, non-transcriptional assay for nuclear receptor ligand binding in yeast.

Gene - 4 Aug 2004

Köhler Fabian, Zimmermann André, Hager Martin, Sippel Albrecht E

Abstract excerpt

We describe the development of a genetic, non-transcriptional assay for the detection of ligand binding to nuclear receptors based on the ligand-dependent reconstitution of the defective Ras/cAMP viability pathway of the Saccharomyces cerevisiae strain CDC25-2. We have characterized the assay using the estrogen receptor (ER) alpha as an example and found it to be extremely sensitive, stringent, rapid and...

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Identifiers

PMID: 15276207
DOI: 10.1016/j.gene.2004.04.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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