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Article

Multiplex single nucleotide polymorphisms genotyping using solid-phase single base extension on magnetic nanoparticles.

Analytical biochemistry - 1 Mar 2009

Liu Hongna, Li Song, Liu Lishang, Tian Lan, He Nongyue

Abstract excerpt

To fulfill the increasing need for large-scale genetic research, we have developed a new solid-phase single base extension (SBE) protocol on magnetic nanoparticles (MNPs) for multiplex SNP detection using adapter polymerase chain reaction (PCR) products as templates. Extension primers were covalently immobilized on the MNPs, and allele-specific extension took place along the stretch of target DNA for one-color...

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PMID: 19100705
DOI: 10.1016/j.ab.2008.11.035

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