Article
MagSNiPer: a new single nucleotide polymorphism typing method based on single base extension, magnetic separation, and chemiluminescence.
Analytical biochemistry - 1 Jun 2005
Kakihara Fumiko, Kurebayashi Yukari, Tojo Yuriko, Tajima Hideji, Hasegawa Setsuo, Yohda Masafumi
Abstract excerpt
We have developed a new method for typing single nucleotide polymorphisms (SNPs), MagSNiPer, based on single base extension, magnetic separation, and chemiluminescence. Single base nucleotide extension reaction is performed with a biotinylated primer whose 3' terminus is contiguous to the SNP site with a tag-labeled ddNTP. Then the primers are captured by magnetic-coated beads with streptavidin, and...
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