Article
In-Situ Mutation Detection by Magnetic Beads-Probe Based on Single Base Extension and Its Application in Genotyping of Hepatitis B Virus Pre-C Region 1896nt Locus Single Nucleotide Polymorphisms.
Journal of biomedical nanotechnology - 1 Dec 2019
Mou Xianbo, Sheng Danni, Chen Zhu, Liu Mei, Liu Yuan, Deng Yan, Xu Kui, Hou Ruixia, Zhao Jiyuan, Zhu Yabin, Li Song
Abstract excerpt
Hepatitis B virus (HBV) is closely related to occurrence and development of viral hepatitis. A mutation of 1896nt locus in its pre-C region can promote replication of HBV DNA and improve stability of pre-genome RNA structure, and can even help HBV evade immune clearance. In this study, magnetic beads-probe (MBs@probe) method, combined with single base extension (SBE) technology, was developed for in-situ mutation...
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