Article
Quantitation of readthrough of termination codons in yeast using a novel gene fusion assay.
Yeast (Chichester, England) - 1 Feb 1991
Firoozan M, Grant C M, Duarte J A, Tuite M F
Abstract excerpt
A simple quantitative in vivo assay has been developed for measuring the efficiency of translation of one or other of the three termination codons. UAA, UAG and UGA in Saccharomyces cerevisiae. The assay employs a 3-phosphoglycerate kinase-beta-galactosidase gene fusion, carried on a multicopy plasmid, in which the otherwise retained reading frame is disrupted by one or other of the three termination codons....
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