Article
Quantitation of readthrough of termination codons in yeast using a novel gene fusion assay.
Yeast (Chichester, England) - 1 Feb 1991
Firoozan M, Grant C M, Duarte J A, Tuite M F
Abstract excerpt
A simple quantitative in vivo assay has been developed for measuring the efficiency of translation of one or other of the three termination codons. UAA, UAG and UGA in Saccharomyces cerevisiae. The assay employs a 3-phosphoglycerate kinase-beta-galactosidase gene fusion, carried on a multicopy pl...
Topics
- Antisense Elements (Genetics)
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Codon
- Molecular Sequence Data
- Mutation
- Peptide Chain Termination, Translational
- Phosphoglycerate Kinase
- Plasmids
- Protein Biosynthesis
- RNA, Transfer, Ser
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Spheroplasts
- Suppression, Genetic
- Transformation, Genetic
- beta-Galactosidase