Article
Up-regulation of tRNA biosynthesis affects translational readthrough in maf1-delta mutant of Saccharomyces cerevisiae.
Current genetics - 1 Dec 2002
Kwapisz Marta, Smagowicz Wiesław J, Oficjalska Danuta, Hatin Isabelle, Rousset Jean-Pierre, Zoładek Teresa, Boguta Magdalena
Abstract excerpt
Maf1p is a negative effector of RNA polymerase III in yeast. The maf1-delta mutation caused an increase in the level of cellular tRNAs, but a decrease of translational readthrough at nonsense codons. Using the lacZ- luc dual gene reporter system, we detected an almost twofold diminution of UAA and UAG readthrough in maf1-delta compared with the parental strain. The maf1-delta mutation did not affect the rate of...
Topics
- Codon, Nonsense
- Codon, Terminator
- Fungal Proteins
- Mutation
- Peptide Termination Factors
- Phenotype
- Prions
- Protein Biosynthesis
- RNA, Transfer
- Saccharomyces cerevisiae