Article
Development of new substrates for high-sensitive genotyping of minority mutated alleles.
Electrophoresis - 1 Dec 2008
Galbiati Silvia, Damin Francesco, Di Carlo Gabriele, Ferrari Maurizio, Cremonesi Laura, Chiari Marcella
Abstract excerpt
An unsurpassed level of sensitivity was reached in the detection of minority mutated alleles. A low-density microarray was printed on a substrate specifically designed to provide an interference effect which amplifies the collection of the light emitted on the support and reinforces the intensity of excitation light. Optimal performance of the array was obtained by maximizing the probe density and the binding...
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