Article
Genotyping of mutation in the beta-globin gene using DNA microarrays.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Dufva Martin, Poulsen Lena
Abstract excerpt
Genotyping using DNA microarrays is a cost-efficient method compared with real-time PCR and DNA sequencing. Here, a DNA microarray-based method using allele-specific oligo hybridization is demonstrated. This method relies on immobilization of probes that are specific for wild-type sequences or the mutated sequences, respectively. The method makes use of agarose film-coated glass slides, unmodified DNA probes,...
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