Article
Detection of mutations using microarrays of poly(C)10-poly(T)10 modified DNA probes immobilized on agarose films.
Analytical biochemistry - 15 May 2006
Dufva Martin, Petersen Jesper, Stoltenborg Michael, Birgens Henrik, Christensen Claus B V
Abstract excerpt
Allele-specific hybridization to a DNA microarray can be a useful method for genotyping patient DNA. In this article, we demonstrate that 13- to 17-base oligonucleotides tagged with a poly(T)10-poly(C)10 tail (TC tag), but otherwise unmodified, can be crosslinked by UV light irradiation to an aga...
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