Article
The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product.
Cell - 19 Oct 1990
Th'ng J P, Wright P S, Hamaguchi J, Lee M G, Norbury C J, Nurse P, Bradbury E M
Abstract excerpt
The mouse cell FT210 was isolated as a G2 phase mutant with a possible defect in the histone H1 kinase. We determined that a temperature-sensitive lesion in this cell line lies in the CDC2 gene. DNA sequence analysis revealed two point mutations in highly conserved regions of the gene: an isoleucine to valine change in the PSTAIR region, and a proline to serine change at the C-terminal region of the protein p34....
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- CDC2 Protein Kinase
- Cell Line
- Enzyme Stability
- G2 Phase
- Kinetics
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed