Mutations in the guanine nucleotide-binding domains of a yeast G alpha protein confer a constitutive or uninducible state to the pheromone response pathway.

Abstract excerpt

Several domains of guanine nucleotide-binding proteins are conserved and form the guanine nucleotide-binding pocket. Mutations in these domains in EF-Tu, ras, and Gas have been shown to result in informative phenotypes. We made several analogous changes in SCG1, which encodes the alpha subunit of the G protein involved in pheromone response in yeast. The scg1Lys388 and scg1Ala391 mutations resulted in severe...

Topics

• Base Sequence
• GTP-Binding Proteins
• Glycine
• Mating Factor
• Molecular Sequence Data
• Mutation
• Peptides
• Phenotype
• Pheromones
• Plasmids
• Saccharomyces cerevisiae