Article
Heterogeneity of expression and secretion of native and mutant [AspB10]insulin in AtT20 cells.
Molecular endocrinology (Baltimore, Md.) - 1 Mar 1991
Ferber S, Gross D J, Villa-Komaroff L, Danehy F, Vollenweider F, Meyer K, Loeken M R, Kahn C R, Halban P A
Abstract excerpt
AtT20 (pituitary corticotroph) cells were transfected with either the native or a mutant [AspB10]rat insulin II gene, using a plasmid containing the insulin gene and a neomycin resistance gene under the control of independent constitutive promoters. The cellular immunoreactive insulin (IRI) content ranged from 0.8-440 ng/10(6) cells, with the highest value similar to that found for a rat insulinoma cell line...
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