Article
Heterogeneity of expression and secretion of native and mutant [AspB10]insulin in AtT20 cells.
Molecular endocrinology (Baltimore, Md.) - 1 Mar 1991
Ferber S, Gross D J, Villa-Komaroff L, Danehy F, Vollenweider F, Meyer K, Loeken M R, Kahn C R, Halban P A
Abstract excerpt
AtT20 (pituitary corticotroph) cells were transfected with either the native or a mutant [AspB10]rat insulin II gene, using a plasmid containing the insulin gene and a neomycin resistance gene under the control of independent constitutive promoters. The cellular immunoreactive insulin (IRI) conte...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- Chromatography, High Pressure Liquid
- Drug Resistance, Microbial
- Gene Expression
- Insulin
- Insulin Secretion
- Mice
- Molecular Sequence Data
- Mutation
- Neomycin
- Pituitary Gland, Anterior
- Plasmids
- RNA, Messenger
- Rats
- Recombinant Proteins
- Transfection