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Article

Sequence requirements for processing of proinsulin in transfected mouse pituitary AtT20 cells.

The Biochemical journal - 1 Sept 1992

Taylor N A, Docherty K

Abstract excerpt

To investigate the sequence requirements for proteolytic processing of prohormones at pairs of basic amino acids, normal and mutant proinsulins were expressed in the mouse pituitary corticotrophic cell line AtT20. The extent of processing was determined by h.p.l.c. analysis of insulin-like immunoreactivity secreted into the media of transfected cells. In this model system, normal proinsulin was efficiently...

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Identifiers

PMID: 1382412
DOI: 10.1042/bj2860619
PMCID: PMC1132943

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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