Article
Sequence requirements for processing of proinsulin in transfected mouse pituitary AtT20 cells.
The Biochemical journal - 1 Sept 1992
Taylor N A, Docherty K
Abstract excerpt
To investigate the sequence requirements for proteolytic processing of prohormones at pairs of basic amino acids, normal and mutant proinsulins were expressed in the mouse pituitary corticotrophic cell line AtT20. The extent of processing was determined by h.p.l.c. analysis of insulin-like immuno...
Topics
- 1-Methyl-3-isobutylxanthine
- Animals
- Cell Line
- Chromatography, High Pressure Liquid
- Insulin
- Mice
- Mutation
- Pituitary Gland
- Pro-Opiomelanocortin
- Proinsulin
- Protein Processing, Post-Translational
- Transfection