Article
Two dominant inhibitory mutants of p21ras interfere with insulin-induced gene expression.
Molecular and cellular biology - 1 Dec 1991
Medema R H, Wubbolts R, Bos J L
Abstract excerpt
Insulin induces a rapid activation of p21ras in NIH 3T3 and Chinese hamster ovary cells that overexpress the insulin receptor. Previously, we suggested that p21ras may mediate insulin-induced gene expression. To test such a function of p21ras more directly, we studied the effect of different domi...
Topics
- 3T3 Cells
- Animals
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Cloning, Molecular
- Gene Expression Regulation
- Genes, Dominant
- HeLa Cells
- Humans
- Insulin
- Mice
- Microbial Collagenase
- Mutation
- Promoter Regions, Genetic
- Proto-Oncogene Proteins p21(ras)
- Receptor, Insulin
- Signal Transduction
- Transfection