Article
Multiplex HLA-typing by pyrosequencing.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Lu Ying, Boehm Julian, Nichol Lynn, Trucco Massimo, Ringquist Steven
Abstract excerpt
Class I and II loci of the human leukocyte antigens (HLA) represent the most polymorphic region of the genome. Evolutionary pressure has resulted in a large number of allelic variants of these loci ensuring the high frequency of heterozygous genotypes observed in human populations. Molecular techniques, including sequencing, are capable of precisely defining HLA alleles. Sequencing by synthesis methodology...
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