Article
Pyrosequencing of clinically relevant polymorphisms.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
King Cristi R, Marsh Sharon
Abstract excerpt
Despite the influx of high throughput sequencing techniques, there is still a niche for low-medium throughput genotyping technologies for small-scale screening and validation purposes. Pyrosequencing is a genotyping assay based on sequencing-by-synthesis. Short runs of sequence around each polymorphism are generated, allowing for internal controls for each sample. Pyrosequencing can also be utilized to identify...
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