Article
Molecular haplotype determination using allele-specific PCR and pyrosequencing technology.
Genomics - 1 Sept 2003
Pettersson Monica, Bylund Mattias, Alderborn Anders
Abstract excerpt
Haplotyping of single-nucleotide polymorphisms (SNPs) is usually performed statistically by computational analysis or by time-consuming cloning techniques. Here we present a simple molecular approach for reliable haplotype determination on individual samples. The procedure is based on allele-specific PCR (AS-PCR) in combination with Pyrosequencing analysis. AS-PCR primers for each allelic variant of the...
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