Article
Single-nucleotide polymorphism analysis by pyrosequencing.
Analytical biochemistry - 10 Apr 2000
Ahmadian A, Gharizadeh B, Gustafsson A C, Sterky F, Nyrén P, Uhlén M, Lundeberg J
Abstract excerpt
There is a growing demand for high-throughput methods for analysis of single-nucleotide polymorphic (SNP) positions. Here, we have evaluated a novel sequencing approach, pyrosequencing, for such purposes. Pyrosequencing is a sequencing-by-synthesis method in which a cascade of enzymatic reactions yields detectable light, which is proportional to incorporated nucleotides. One feature of typing SNPs with...
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