Article
Pyrosequence-based typing of alleles of the HLA-DQB1 gene.
BioTechniques - 1 Jul 2002
Ringquist S, Alexander A M, Rudert W A, Styche A, Trucco M
Abstract excerpt
DNA typing of alleles of the highly polymorphic HLA-DQBI gene was performed by Pyrosequencing using purified DNA from the 11th International Histocompatibility Workshop human cell lines and samples from the Children's Hospital of Pittsburgh registry of diabetics and their first-degree relatives. Pyrosequencing was optimized for genotyping exon 2 of the HLA-DQB1 gene, but the procedure should be applicable to...
Topics
- Alleles
- Base Sequence
- Cell Line, Transformed
- Computer Simulation
- DNA Mutational Analysis
- Diabetes Mellitus
- Genome, Human
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Hematopoietic Stem Cells
- Humans