Article
Energetic contributions to the initiation of transcription in E. coli.
Biophysical chemistry - 1 Dec 2008
Ramprakash Jayanthi, Schwarz Frederick P
Abstract excerpt
The thermodynamics of RNA polymerase (RNAP) binding to a 108 base pair (bp) synthetic promoter with consensus sequences at the -35 and -10 bp binding regions upstream from the transcription start point were determined using isothermal titration calorimetry (ITC). The binding constant at 25 degrees C is 2.37+/-0.18x10(7) M(-1), which is reduced to 0.17+/-0.06x10(7) M(-1) with mutations in the -10 bp region but...
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