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Article

Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases.

The Journal of biological chemistry - 29 Apr 2005

Schroeder Lisa A, deHaseth Pieter L

Abstract excerpt

Formation of strand-separated, functional complexes at promoters was compared for RNA polymerases from the mesophile Escherichia coli and the thermophile Thermus aquaticus. The RNA polymerases contained sigma factors that were wild type or bearing homologous alanine substitutions for two aromatic amino acids involved in DNA melting. Substitutions in the sigmaA subunit of T. aquaticus RNA polymerase impair...

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Identifiers

PMID: 15731103
DOI: 10.1074/jbc.M501281200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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