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Article

Next generation sequencing-based parallel analysis of melting kinetics of 4096 variants of a bacterial promoter.

Biochemistry - 21 Jan 2014

Heyduk Ewa, Heyduk Tomasz

Abstract excerpt

Promoter melting by bacterial RNA polymerase is a key step in transcription initiation. We used a next generation sequencing (NGS) based approach to analyze in parallel promoter melting of all 4096 sequence variants of the 6 bp -10 promoter element. We used NGS read count for each sequence of a promoter library containing a randomized -10 sequence as an observable to determine relative enrichment of -10 element...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24359527
DOI: 10.1021/bi401277w
PMCID: PMC3931131

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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