Article
High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain.
Applied and environmental microbiology - 1 Nov 2008
Zhu Yihui, Eiteman Mark A, Altman Ronni, Altman Elliot
Abstract excerpt
We report pyruvate formation in Escherichia coli strain ALS929 containing mutations in the aceEF, pfl, poxB, pps, and ldhA genes which encode, respectively, the pyruvate dehydrogenase complex, pyruvate formate lyase, pyruvate oxidase, phosphoenolpyruvate synthase, and lactate dehydrogenase. The glycolytic rate and pyruvate productivity were compared using glucose-, acetate-, nitrogen-, or phosphorus-limited...
Topics
- Acetic Acid
- Enzymes
- Escherichia coli
- Escherichia coli Proteins
- Glucose
- Glycolysis
- Metabolic Networks and Pathways
- Models, Biological
- Mutation
- Nitrogen
- Phosphorus