Identification of Enzymatic Bottlenecks for the Aerobic Production of Malate from Glycerol by the Systematic Gene Overexpression of Anaplerotic Enzymes in Escherichia coli.

Abstract excerpt

The biotechnological production of dicarboxylic acids (C4) from renewable carbon sources represents an attractive approach for the provision of these valuable compounds by green chemistry means. Glycerol has become a waste product of the biodiesel industry that serves as a highly reduced carbon source for some microorganisms. Escherichia coli is capable of consuming glycerol to produce succinate under anaerobic...

Topics

• Aerobiosis
• Bicarbonates
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Glycerol
• Kinetics
• Malate Dehydrogenase
• Malates
• Mutation
• Phosphoenolpyruvate Carboxylase