Article
Limited functional equivalence of phylogenetic variation in small nuclear RNA: yeast U2 RNA with altered branchpoint complementarity inhibits splicing and produces a dominant lethal phenotype.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1991
Miraglia L, Seiwert S, Igel A H, Ares M
Abstract excerpt
U2 is a highly conserved small nuclear RNA essential for pre-mRNA splicing in mammals and yeast and for trans-splicing in trypanosomes. To test the function of variant U2 RNA structures from different organisms, we conducted phylogenetic exchanges of U2 domains. Replacing nucleotides 1-120 of yea...
Topics
- Base Sequence
- Genes, Dominant
- Genes, Fungal
- Genes, Lethal
- Genetic Variation
- Humans
- Hydrogen Bonding
- Models, Structural
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Oligonucleotide Probes
- Phenotype