Article
Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element.
Genes & development - 1 Dec 1991
Zavanelli M I, Ares M
Abstract excerpt
To understand the role of U2 RNA structure in pre-mRNA splicing we have characterized several cold-sensitive mutations in an essential stem-loop of yeast U2. Although mutant U2 is stable in vivo after a shift to restrictive temperature, splicing is rapidly inhibited, suggesting a direct effect on U2 function rather than U2 synthesis or snRNP assembly. Splicing complexes form at 23 degrees C in both mutant and...
Topics
- Base Sequence
- Cold Temperature
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Phenotype
- RNA Precursors
- RNA Splicing
- RNA, Fungal
- RNA, Small Nuclear
- Ribonucleoproteins