Article
Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.
Molecular biology of the cell - 1 Oct 2008
Cohen R L, Espelin C W, De Wulf P, Sorger P K, Harrison S C, Simons K T
Abstract excerpt
Mif2p is the budding-yeast orthologue of the mammalian centromere-binding protein CENP-C. We have mapped domains of Saccharomyces cerevisiae Mif2p and studied the phenotyptic consequences of their deletion. Using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays, we have further shown that Mif2p binds in the CDEIII region of the budding-yeast centromere, probably in close spatial...
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