Recovery of DNA sequences flanking P-element insertions in Drosophila: inverse PCR and plasmid rescue.

doi:10.1101/pdb.prot5199

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Article

Recovery of DNA sequences flanking P-element insertions in Drosophila: inverse PCR and plasmid rescue.

Cold Spring Harbor protocols - 1 Apr 2009

Huang Audrey M, Rehm E Jay, Rubin Gerald M

Abstract excerpt

The Drosophila melanogaster P-transposable element is a powerful and widely used research tool. Sequences flanking the P-element can be recovered and the site of insertion can be mapped to the nucleotide, to connect the genetic and physical maps and facilitate molecular analysis of the gene of interest. The Berkeley Drosophila Genome Project (BDGP) has assembled a well-characterized collection of lethal mutations...

Topics

Animals
Chromosome Mapping
DNA
DNA Transposable Elements
Drosophila
Genetic Techniques
Mutation
Nucleotides
Plasmids
Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 20147142
DOI: 10.1101/pdb.prot5199

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