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Article

Screening Mutants by Single Fly Genomic PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022

Nagarajan Usha, Georgiou Marios

Abstract excerpt

Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confirmed. Here, we provide a method to detect and confirm the presence of a mutation, as and when mutant flies are generated. By allowing for the maintenance and...

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PMID: 35674888
DOI: 10.1007/978-1-0716-2201-8_2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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