Article
High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer.
American journal of clinical pathology - 1 Aug 2008
Simi Lisa, Pratesi Nicola, Vignoli Marina, Sestini Roberta, Cianchi Fabio, Valanzano Rosa, Nobili Stefania, Mini Enrico, Pazzagli Mario, Orlando Claudio
Abstract excerpt
High-resolution melting analysis (HRMA) provides a valid approach to efficiently detect DNA genetic and somatic mutations. In this study, HRMA was used for the screening of 116 colorectal cancers (CRCs) to detect hot-spot mutations in the KRAS and BRAF oncogenes. Mutational hot spots on the PIK3CA gene, exons 9 and 20, were also screened. Direct sequencing was used to confirm and characterize HRMA results. HRMA...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.