Article
A missense mutation in the rpoC gene affects chromosomal replication control in Escherichia coli.
Journal of bacteriology - 1 Aug 1991
Petersen S K, Hansen F G
Abstract excerpt
An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is increased twofold in the mutant at semipermissive growth temperatures (39 degrees C). In a delta...
Topics
- Aspartic Acid
- Bacterial Proteins
- Chromosomes, Bacterial
- DNA Replication
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Glycine
- Immunoblotting