Article
Use of a DNA polymerase III bypass mutant of Escherichia coli, pcbA1, to isolate potentially useful mutations of a complex plasmid replicon.
Plasmid - 1 Jan 1996
Banerjee S, Kim H Y, Iyer V N
Abstract excerpt
The essential replicon region of plasmid pCU1 has, within 1.2 kb, two origins of replication that can function in the absence of Escherichia coli DNA polymerase I and one that requires this polymerase. To isolate mutants in the replicon pathway that uses the PolI-dependent origin in the presence...
Topics
- Base Sequence
- DNA Polymerase III
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Replicon
- Temperature