Article
The role of transcriptional activator GATA-1 at human beta-globin HS2.
Nucleic acids research - 1 Aug 2008
Cho Youngran, Song Sang-Hyun, Lee Jong Joo, Choi Narae, Kim Chul Geun, Dean Ann, Kim AeRi
Abstract excerpt
GATA-1 is an erythroid activator that binds beta-globin gene promoters and DNase I hypersensitive sites (HSs) of the beta-globin locus control region (LCR). We investigated the direct role of GATA-1 interaction at the LCR HS2 enhancer by mutating its binding sites within minichromosomes in erythroid cells. Loss of GATA-1 in HS2 did not compromise interaction of NF-E2, a second activator that binds to HS2, nor was...
Topics
- Acetylation
- Binding Sites
- CREB-Binding Protein
- Deoxyribonuclease I
- Enhancer Elements, Genetic
- GATA1 Transcription Factor
- Globins
- Histones
- Humans
- K562 Cells
- Locus Control Region