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Article

Optimized generation of vectors for the construction of Haloferax volcanii deletion mutants.

Journal of microbiological methods - 1 Oct 2008

Hammelmann M, Soppa Jörg

Abstract excerpt

A method for the construction of in frame deletions in chromosomal genes of the archaeon Haloferax volcanii has been described recently (Allers et al. (2004), Appl. Environ. Microbiol. 70:943-953). Two steps of the procedure for deletion vector construction were optimized. First, the deletion version of the gene of interest was generated by fusion of two PCR products, which were comprised of the upstream and...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 18582505
DOI: 10.1016/j.mimet.2008.05.029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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