Article
The extremely halophilic archaeon Haloferax volcanii has two very different dihydrofolate reductases.
Molecular microbiology - 1 Mar 2000
Ortenberg R, Rozenblatt-Rosen O, Mevarech M
Abstract excerpt
The gene encoding dihydrofolate reductase, hdrA, from the extremely halophilic archaeon Haloferax volcanii was previously isolated from a spontaneous trimethoprim-resistant mutant in a DNA sequence that had undergone amplification. Here, we show that deletion of hdrA did not affect growth in minimal medium and that the strain carrying the deletion remained sensitive to trimethoprim. A spontaneous...
Read the complete abstract on PubMedTopics
- Amino Acid Sequence
- Archaeal Proteins
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA Transposable Elements